The well-known problem of the stability of the dormant state of tumor cells, which can be activated and begin active reproduction due to a still unknown mechanism, is considered. It has been shown that such a mechanism can be a threshold change in the intensity of the processes of spatial migration of these tumor cells, for example, during invasive diagnostic interventions in clinical practice. The hypothetical prototypical human malignant tumor model was created for this study based on our own long-time fundamental theoretical, experimental, and clinical research on ultra-low temperatures in biomedical science and its practical application within surgical oncology concerning diagnostics and prevention of malignant diseases. Numerous animal and clinical studies in vitro and in vivo were published in the world scientific professional literature point by point previously using active-passive freezing-thawing cycles under ultra-low temperatures. A discrete set of ranges of changes in the values of the diffusion coefficients, which determines the intensity of cell migration, in which diffusion instability of tumor cell dormancy occurs, has been established. In this study, experimental and clinical investigations in vitro and in vivo paved the way for a mathematical estimation of the standard tumor diagnostics and cryodiagnostics in living biological matter. The use of an ultra-low-temperature technique for carrying out standard diagnostic procedures in surgical oncology is proposed, which can prevent the risk of occurrence of diffusion instability of the tumor dormant state.

Nikolai N. Korpan教授丨第二十一届世界冷冻大会发言：《肿瘤休眠状态的扩散不稳定性和标准的介入性肿瘤诊断》

我们考虑了众所周知的肿瘤细胞休眠状态的稳定性问题，由于一种仍然未知的机制，肿瘤细胞可以被激活并开始积极繁殖。事实证明，这种机制可以是这些肿瘤细胞空间迁移过程强度的阈值变化，例如，在临床实践中的侵入性诊断干预期间。假设的人类恶性肿瘤原型模型是基于我们自己对生物医学中超低温的长期基础理论、实验和临床研究及其在肿瘤外科中关于诊断和预防恶性疾病的实际应用而建立的。以前在世界科学专业文献中，利用超低温下的主动-被动冷冻-解冻循环，在体外和体内进行了大量的动物和临床研究。目前已经建立了一套离散的扩散系数值的变化范围，它决定了细胞迁移的强度，在这种情况下，肿瘤细胞休眠的扩散不稳定性会发生。在这项研究中，体外和体内的实验和临床调查为活体生物物质的标准肿瘤诊断和冷冻诊断的数学估计铺平了道路。提出使用超低温技术进行肿瘤外科的标准诊断程序，可以防止肿瘤休眠状态的扩散不稳定发生的风险。